Now Available in
Auckland, New Zealand
PacBio Revio HiFi: long‑read sequencing at scale for the most affordable HiFi genomes yet. With new SPRQ chemistry, Revio delivers richer, multiomic data with lower price per genome, making highly accurate long reads practical for large studies.
Now Available in Auckland, New Zealand.
Why choose Revio HiFi
Revio is a long‑read sequencing system designed to provide high‑throughput, highly accurate HiFi data for whole genomes, targets, epigenetics, and RNA on a single platform. HiFi reads from Revio offer long read lengths with 99.9% accuracy and uniform coverage, enabling reference‑quality assemblies and comprehensive variant detection. The system integrates powerful onboard compute, streamlined consumables, and new SPRQ‑Nx chemistry to reduce costs and add multiomic capabilities.
What does Revio offer?
Access more information at reduced cost and higher throughput, generating affordable HiFi genomes for large cohorts and population‑scale projects. Consolidate multiple assays into a single HiFi workflow that captures small variants, structural variants, repeat expansions, methylation, and full‑length transcripts. Benefit from highly accurate long reads with exceptional consensus accuracy and uniform coverage across the genome, even in hard‑to‑sequence regions. Enable multiomic studies with direct epigenetic detection and RNA sequencing on the same platform, powered by updated chemistries and application‑specific kits.
Applications
- Whole genome sequencing: achieve the highest consensus accuracy and uniform coverage for reference‑quality genomes in humans, plants, animals, and microbes.
- Targeted sequencing: accurately detect all variant types, including difficult repeat expansions and structural variants, even in hard‑to‑reach genomic regions.
- Epigenetics: explore how epigenetic changes, including DNA methylation, affect gene expression, host-pathogen interactions, and environmental response using native DNA.
- RNA sequencing: discover new genes, transcripts, and alternative splicing events with full‑length isoform sequencing, including high‑throughput workflows using the Kinnex full‑length RNA kit.
Revio long‑read sequencing enables you to access more genomic and epigenomic information at reduced cost and higher throughput on a single, integrated system.
From whole genomes and targeted panels to epigenetics and RNA, there's a HiFi solution for the questions that matter most in your research.
Papers of Note
Clinical long-read genome sequencing for rare disease diagnostics(external link)
Tessa J.J. de Bitter et al doi: https://doi.org/10.64898/2026.01.13.26343759
Background Diagnostic evaluation of rare genetic disorders continues to rely on multiple test modalities, despite the increasing use of short-read exome or genome sequencing as first-tier tests. Long-read genome sequencing (lrGS) has the potential to consolidate current standard-of-care (SoC) diagnostics into a single assay, but its accuracy and clinical utility in routine practice have not been established at large scale.
Methods We studied 1000 clinical samples, including 832 index cases, representative of one year
Population-scale Long-read Sequencing in the All of Us Research Program(external link)
Kiran V Garimelladoi et al: https://doi.org/10.1101/2025.10.02.25336942
The All of Us Research Program (AoU) is a national biobank seeking to enroll one million individuals in the United States to link genomic and biomedical data, including short- and long-read whole-genome sequencing (srWGS/LRS), with rich electronic health record (EHR) information. Here, we present the first large-scale analyses of long-read sequencing (LRS) in AoU and offer a new framework for deriving genomic insights into complex structural variation (SV) of relevance to human health and disease. We performed joint analyses of 1,027 individuals self-identifying as Black or African American, sequenced to ∼8x coverage with Pacific Biosciences HiFi technology and processed using cloud-native pipelines. From these LRS data we constructed a comprehensive variant callset encompassing known (FMR1 and HTT) and novel repeat expansions, clinically relevant haplotypes at loci inaccessible to srWGS, and haplotypes relevant to disease risk (HLA) and pharmacogenomics (CYP2D6), including SNVs, indels, and SVs. We developed methods for cohort-level variant calling and...
Haplotype-resolved diverse human genomes and integrated analysis of structural variation. (external link)
Ebert et al. Science 2021 Apr 2;372(6537):eabf7117. doi: 10.1126/science.abf7117.
Long-read and strand-specific sequencing technologies together facilitate the de novo assembly of high-quality haplotype-resolved human genomes without parent-child trio data. We present 64 assembled haplotypes from 32 diverse human genomes. These highly contiguous haplotype assemblies (average minimum contig length needed to cover 50% of the genome: 26 million base pairs) integrate all forms of genetic variation, even across complex loci. We identified 107,590 structural variants (SVs), of which 68% were not discovered with short-read sequencing, and 278 SV hotspots (spanning megabases of gene-rich sequence).....
Single-Cell Long-Read Sequencing Resolves Isoform Diversity in Human Brain Cells(external link)
Jogelkar et al. Nature Neuroscience, volume 27, pages1051–1063 (2024)
RNA isoforms influence cell identity and function. However, a comprehensive brain isoform map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes, developmental time points and species. For 72% of genes, full-length isoform expression varies along one or more axes. Splicing, transcription start and polyadenylation sites vary strongly between cell types, influence protein architecture and associate with disease-linked variation. Additionally, neurotransmitter transport and synapse turnover genes harbor cell-type variability across anatomical regions.
High-quality metagenome assembly from long accurate reads with metaMDBG(external link)
Benoit et al. Nature Biotechnology volume 42, pages1378–1383 (2024)
...Third-generation long-read sequencing technologies have greatly improved the quality of metagenome assemblies and MAGs. The first applications, using reads generated by the Oxford Nanopore Technologies (ONT) platform (which, at that point, had a relatively high error rate) typically only resolved a small fraction of the community as complete circularized contigs7. More recent ONT studies have generated hundreds of MAGs but only a relatively small number of closed circularized genomes8,9. An alternative long-read technology, HiFi PacBio, combines long reads with very high accuracies (≈99.9%). This has enabled hundreds of MAGs to be retrieved from metagenomes with a substantially larger fraction as circularized contigs10....
Culture-Independent Meta-Pangenomics Enabled by Long-Read Metagenomics Reveals Novel Associations with Pediatric Undernutrition(external link)
Cell Press J. Minich et al. 20 Sep 2024
In shotgun metagenomic sequencing of human fecal samples, PacBio outperforms ONT in read quality, mean / median read lengths, N50 read lengths, and accuracy. PacBio cMAGs have significantly higher completeness and lower contamination than ONT at any depth. PacBio HiFi recovers more MAGs than ONT at the same number of total data, regardless of metagenome assembler used. PacBio obtained the same number of cMAGs as ILMN using just 1.4% of data. Synthetic long reads (TELL-Seq) did not yield any cMAGs from 625 Gbp of data. PacBio detected the most significant associations with metadata, had the fewest unclassified reads, and improved taxonomic profiling classification rates by 68%.
